polyclonal antibodies against cd133  (St Johns Laboratory)

Journal: Cardiovascular Diagnosis and Therapy

Article Title: A new method of culturing rat bone marrow endothelial progenitor cells in vitro

doi: 10.21037/cdt-20-536

Figure Lengend Snippet: Immunocytochemical analysis of BMMNCs. After 14 days of culture, BMMNCs revealed expression of rat stem cell markers (×400). (A) CD34 is positive and the cytoplasm is brown (86.36±5.12); (B) CD45 is positive (30.25±4.62); (C) CD133 is positive (19.72±5.20).

Article Snippet: We used primary rabbit polyclonal antibodies against rat CD133 (BioTech, Beijing, China) and rat VEGFR2 (Boster, Wuhan, China).

Techniques: Expressing

Journal: Cardiovascular Diagnosis and Therapy

Article Title: A new method of culturing rat bone marrow endothelial progenitor cells in vitro

doi: 10.21037/cdt-20-536

Figure Lengend Snippet: Flow cytometry analysis of BMMNCs differentiating into EPCs. After 12 days in vitro, the results of flow cytometry show that: (A) CD34 is positive (89.0%); (B) CD45 is positive (83.2%); (C) CD133 is positive (75.5%). BMMNCs, bone-marrow mononuclear cells; EPCs, endothelial progenitor cells.

Article Snippet: We used primary rabbit polyclonal antibodies against rat CD133 (BioTech, Beijing, China) and rat VEGFR2 (Boster, Wuhan, China).

Techniques: Flow Cytometry, In Vitro

Journal: Lung Cancer Management

Article Title: Targeting claudin-3 suppresses stem cell-like phenotype in nonsquamous non-small-cell lung carcinoma

doi: 10.2217/lmt-2018-0010

Figure Lengend Snippet: (A) Expression analysis of ER-α and claudin-3 in AC and SCC samples from GEO database (GDS3627). (B) Effect of ER-α knockdown on expression of claudin-3 as well as CSCs markers including CD133, CD24, ABCG2 in the breast cancer cell line MCF7. Data were obtained from GEO database (GDS4061). (C) Effect of ER-α knockdown on the claudin-3 protein level in H1792. (D) Effect of ER-α knockdown on claudin-3 transcription in H1792. (E) Effect of ER-α and claudin-3 double knockdown on ER-α or claudin-3 expression in H460. (F) Effect of ER-α and claudin-3 double knockdown on the percentage of SP in H460. Data from triplicates are presented as the mean ± standard deviation; **p < 0.01;***p < 0.001. CSC: Cancer stem-like cell; ER-α: Estrogen receptor-α; GEO: Gene Expression Omnibus; SCC: Squamous cell lung carcinoma; SP: Side-population.

Article Snippet: Mouse monoclonal antibody against ER-α (catalog number GTX70171) and rabbit polyclonal antibodies against CD133 (catalog number GTX12295), ALDH1A1 (catalog number GTX123974) and ABCG2 (catalog number GTX100437) were purchased from GeneTex (CA, USA).

Techniques: Expressing, Standard Deviation

Journal: PLoS ONE

Article Title: Suppression of cancer stemness by upregulating Ligand-of-Numb protein X1 in colorectal carcinoma

doi: 10.1371/journal.pone.0188665

Figure Lengend Snippet: (A) Sorting of SP and non-SP cells using BD Aria software. The verapamil group was set as the negative control. (B) and (C) are the western blot and the semi-quantitative RT PCR analysis of genes (LNX1 as well as CSC markers in CRC including LGR5, CD133, ABCB5 and ALDH1A1).

Article Snippet: Rabbit polyclonal antibodies against ALDH1A1, CD133, LGR5 and the mouse monoclonal antibody against ABCB5 were purchased from GeneTex (GeneTex, CA).

Techniques: Software, Negative Control, Western Blot, Quantitative RT-PCR

Journal: Oncotarget

Article Title: Intravascular emboli is an independent risk factor for the prognosis of stage III colorectal cancer patients after radical surgery

doi: 10.18632/oncotarget.11266

Figure Lengend Snippet: Correlations among intravascular emboli (IVE), CD133 expression and clinicopathological characteristics.

Article Snippet: After blocking with 3% BSA, the sections were incubated with rabbit polyclonal antibody against human CD133 at a dilution of 1:200 (18470-1-AP, Proteintech, China) overnight at 4°C, and this was followed by incubation with secondary antibody for 50 minutes at room temperature.

Techniques: Expressing

Journal: Oncotarget

Article Title: Intravascular emboli is an independent risk factor for the prognosis of stage III colorectal cancer patients after radical surgery

doi: 10.18632/oncotarget.11266

Figure Lengend Snippet: A , B . Positive expression of CD133 in IVE colorectal cancer tissue: CD34 IHC staining marked the blood vessel endothelium (red arrow), and IVE was strongly stained by CD133 antibody (black arrow); C , D . CD133-negative expression in IVE colorectal cancer tissue: CD34 IHC staining marked the blood vessel endothelium (red arrow); IVE was not stained by the CD133 antibody (black arrow); E . Positive expression of CD133 in non-IVE colorectal cancer tissue (black arrow); F. CD133-negative expression in non-IVE colorectal cancer tissue (black arrow).

Article Snippet: After blocking with 3% BSA, the sections were incubated with rabbit polyclonal antibody against human CD133 at a dilution of 1:200 (18470-1-AP, Proteintech, China) overnight at 4°C, and this was followed by incubation with secondary antibody for 50 minutes at room temperature.

Techniques: Expressing, Immunohistochemistry, Staining

Journal: Oncotarget

Article Title: Intravascular emboli is an independent risk factor for the prognosis of stage III colorectal cancer patients after radical surgery

doi: 10.18632/oncotarget.11266

Figure Lengend Snippet: Univariate and multivariate analysis of 323 CRC patients for OS and DFS

Article Snippet: After blocking with 3% BSA, the sections were incubated with rabbit polyclonal antibody against human CD133 at a dilution of 1:200 (18470-1-AP, Proteintech, China) overnight at 4°C, and this was followed by incubation with secondary antibody for 50 minutes at room temperature.

Techniques: Expressing